Humanin
Also known as: HN, Humanin-G
A mitochondrial-derived peptide with potent neuroprotective and anti-apoptotic properties. Naturally produced in the body and associated with longevity and metabolic health.
Research Status
Preclinical/Research Phase
For research purposes only. Not approved for human use. Not medical advice.
Research Areas
Side Effects
Redness, mild swelling, or itching at injection site. Usually resolves within hours. Minimize by rotating sites, allowing solution to reach room temperature, and using proper injection technique.
Mild headache reported in some users. Typically transient. Ensure adequate hydration and monitor timing relative to injections.
Transient facial flushing reported in isolated cases. Self-resolving and does not require intervention.
Mild nausea reported in very few users. May be dose-dependent. Consider taking with food or reducing dose if persistent.
Localized fat loss or thickening at repeated injection sites. Preventable through strict site rotation (minimum 1 inch between sites, cycle through 4-6+ sites). Discontinue injections at affected sites until resolved.
Dosing Reference
| Parameter | Value |
|---|---|
| Dose range | 1-5 mg |
| Frequency | 1x daily |
| Timing | Any time of day |
| Route | Topical |
Research compound. Various analogs exist (HNG, HNGF6A) with enhanced potency. Dosing based on in vitro and animal model studies; human clinical data limited. Start at lower end and assess response.
Research disclaimer
Figures drawn from published research literature and community logs. Not clinical recommendations. Consult a qualified professional. Research use only.
Reconstitution Guide
Do not use saline or bacteriostatic saline — use only bacteriostatic water for reconstitution
Do not shake the vial vigorously; gentle swirling prevents peptide degradation
Discard immediately if the solution appears cloudy, discolored, or contains visible particles
Use within 30 days of reconstitution when stored at 2–8°C
Do not freeze the reconstituted solution; freezing may denature the peptide
Use the PeptideVolt reconstitution calculator for your exact concentration
Molecular and Pharmacological Data
| Molecular weight | 2174 |
| Sequence | MAPRGFLSCLLLTGC |
Humanin is a mitochondrial-derived peptide that activates multiple cytoprotective signaling pathways, including JAK/STAT and PI3K/Akt, to suppress apoptosis and enhance cellular stress resistance. It improves mitochondrial function and bioenergetics, reducing oxidative stress and inflammation while enhancing metabolic health and neuroprotection. These mechanisms underlie its reported effects on longevity, cognitive function, and metabolic regulation.
JAK/STAT Signaling
Humanin activates JAK/STAT pathway to suppress pro-apoptotic signals and enhance cell survival, particularly in neurons and immune cells
PI3K/Akt/mTOR Pathway
Promotes cell survival and metabolic anabolism through Akt phosphorylation, enhancing glucose uptake and insulin sensitivity
Mitochondrial Bioenergetics
Enhances ATP production and mitochondrial oxidative capacity, reducing ROS generation and cellular energy stress
NF-κB Inhibition
Suppresses pro-inflammatory NF-κB signaling, reducing systemic inflammation and neuroinflammation
TrkA/p75NTR Signaling
Interacts with neurotrophic receptor pathways to enhance neuroprotection and neuronal survival
- Humanin is endogenously produced in mitochondria and circulates systemically; levels decline with age
- Acts as a ligand for multiple receptors including FPRL1, TrkA, and formyl peptide receptors
- Demonstrates neuroprotection against Alzheimer's disease pathology and other neurodegenerative insults in preclinical models
- Enhances insulin signaling and glucose metabolism in both in vitro and animal models
- Synthetic analogs (HNG, HNGF6A) show enhanced potency and stability compared to native humanin
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View all peptidesResearch Use Only. All content on this page is provided for informational and educational purposes related to scientific research. Humanin is not approved for human use by the FDA or any equivalent regulatory body. This is not medical advice. Do not use any substance discussed here for therapeutic, diagnostic, or preventative purposes. Consult a qualified healthcare professional before making any health-related decisions. The Peptide Volt does not endorse the use of any research chemicals. 18+ only.