TB-500
Also known as: Thymosin Beta-4, Tβ4
TB-500 is a synthetic version of Thymosin Beta-4, a naturally occurring peptide present in high concentrations in blood platelets, wound fluid, and other tissues. It plays a crucial role in tissue repair and regeneration.
Research Status
Research compound with veterinary use history
For research purposes only. Not approved for human use. Not medical advice.
Research Areas
Side Effects
Usually resolves within 1–2 hours. Minimize by allowing the solution to reach room temperature before injection, rotating injection sites, and using proper aseptic technique. Apply ice if swelling persists.
Reported in some users, typically mild and self-resolving. May be related to increased blood flow or immune activation. Ensure adequate hydration and monitor frequency.
Rare but reported; may occur immediately after injection. Sit or lie down if this occurs. Ensure you are well-hydrated and have eaten before injection.
Transient vasodilation may cause temporary facial redness or warmth. Typically resolves within minutes. Not medically concerning but may be cosmetically noticeable.
Uncommon; if it occurs, it is usually mild and transient. Ensure adequate food intake before injection and maintain hydration.
True IgE-mediated allergic reactions are rare but possible. Discontinue use immediately if rash, hives, facial swelling, or difficulty breathing occur. Seek emergency medical attention for anaphylaxis. Individuals with known peptide sensitivities should consult a healthcare provider before use.
Caused by repeated injections in the same area. Prevent by rotating injection sites systematically with each dose. Space injections at least 1 inch apart. If lipodystrophy develops, discontinue injections in that area and allow 4–8 weeks for recovery.
Risk is minimized by using sterile technique, clean needles, and proper site preparation. Signs include increasing redness, warmth, swelling, pus, or fever. Seek medical attention if infection is suspected.
Transient increase in heart rate may occur due to immune activation or increased blood flow. Monitor vital signs if this occurs. Seek medical attention if heart rate remains elevated or is accompanied by chest pain or shortness of breath.
Dosing Reference
| Parameter | Value |
|---|---|
| Dose range | 2-2.5 mg |
| Frequency | 2x/week (loading phase) then 1x/week (maintenance) |
| Timing | Flexible timing - morning or evening |
| Route | Subcutaneous, Intramuscular |
Loading phase: 2-2.5mg twice weekly for 4-6 weeks. Maintenance: once weekly or as needed.
Research disclaimer
Figures drawn from published research literature and community logs. Not clinical recommendations. Consult a qualified professional. Research use only.
Reconstitution Guide
Do not use saline or bacteriostatic saline — use only bacteriostatic water for reconstitution
Do not shake the vial vigorously; gentle swirling prevents peptide degradation
Discard immediately if the solution appears cloudy, discolored, or contains visible particles
Use within 30 days of reconstitution when stored at 2–8°C
Do not freeze the reconstituted solution; freezing may denature the peptide
Use the PeptideVolt reconstitution calculator for your exact concentration
Molecular and Pharmacological Data
| Molecular weight | 949.97 Da (for SDKP fragment); full TB-500 analog approximately 5000 Da |
| Half-life | Approximately 2 hours in circulation (short half-life; frequent dosing or depot formulations may be considered) |
| Sequence | SDKP (Ser-Asp-Lys-Pro) — the active N-terminal tetrapeptide fragment; full thymosin beta-4 is 43 amino acids |
TB-500 is a synthetic analog of thymosin beta-4, an endogenous peptide involved in tissue repair and regeneration. It promotes cell migration, angiogenesis (new blood vessel formation), and anti-inflammatory responses, facilitating healing of damaged tissues including muscle, tendon, ligament, and skin. The peptide works by upregulating actin polymerization and modulating growth factor signaling pathways essential for tissue remodeling and recovery.
Actin Polymerization and Cell Migration
TB-500 binds to actin monomers and regulates their polymerization, which is essential for cell motility and migration. This mechanism allows immune cells and fibroblasts to migrate to damaged tissue sites more efficiently, accelerating the inflammatory and repair phases of wound healing.
Angiogenesis (New Blood Vessel Formation)
TB-500 promotes the growth of new blood vessels by stimulating endothelial cell proliferation and migration. Improved blood flow to injured tissues enhances oxygen and nutrient delivery, supporting faster tissue regeneration and reducing healing time.
Anti-inflammatory Signaling
TB-500 modulates inflammatory cytokine production and immune cell activity, reducing excessive inflammation while maintaining the necessary inflammatory response for proper tissue repair. This balance prevents chronic inflammation that can impair healing.
Growth Factor Upregulation
TB-500 increases expression of growth factors such as vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF), which are critical for tissue regeneration, cell survival, and differentiation.
- TB-500 is derived from thymosin beta-4, a naturally occurring peptide found in high concentrations in blood platelets, immune cells, and wound fluid
- The peptide's primary mechanism involves regulating actin dynamics, which is fundamental to cell migration and tissue remodeling
- TB-500 promotes both angiogenesis and anti-inflammatory responses, creating an optimal environment for tissue healing
- Research demonstrates efficacy in accelerating recovery from muscle strains, tendon injuries, and wound healing in animal models and some clinical studies
- The peptide has a relatively short circulating half-life, necessitating regular dosing schedules (typically twice weekly during loading phase)
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View all peptidesResearch Use Only. All content on this page is provided for informational and educational purposes related to scientific research. TB-500 is not approved for human use by the FDA or any equivalent regulatory body. This is not medical advice. Do not use any substance discussed here for therapeutic, diagnostic, or preventative purposes. Consult a qualified healthcare professional before making any health-related decisions. The Peptide Volt does not endorse the use of any research chemicals. 18+ only.