Research Use Only - Not for human consumption. 18+ only.
Clinical TrialsCognitive Enhancement / NeuroprotectionOral

NSI-189

Also known as: NSI-189 phosphate

NSI-189 is a benzylpiperazine-aminopyridine small-molecule compound that promotes hippocampal neurogenesis and increases hippocampal volume. Originally developed as a potential antidepressant, it has demonstrated cognitive enhancement and neuroprotective properties in clinical research. NSI-189 is not a peptide but a small-molecule compound; however, it is commonly tracked in research compound databases. It is administered orally and has completed Phase 2 clinical trials.

Research Status

Clinical Trials

Limited Clinical Data

For research purposes only. Not approved for human use. Not medical advice.

Research Areas

Promotes hippocampal neurogenesis
Increases hippocampal volume
Enhances cognitive function and memory
Supports neuroprotection
May improve mood and depression symptoms
Supports brain-derived neurotrophic factor (BDNF) signaling
Potential antidepressant effects

Side Effects

Nausea and gastrointestinal upset
CommonMild

Most common in early treatment. Taking NSI-189 with food significantly reduces incidence. Usually resolves within 1-2 weeks of continued use.

Headache
UncommonMild

Reported in clinical trials. Typically mild and self-resolving. Ensure adequate hydration.

Dizziness or lightheadedness
UncommonMild

May occur early in treatment. Avoid sudden position changes. Usually resolves with continued use.

Insomnia or sleep disturbance
UncommonMild

Some users report sleep changes. Taking NSI-189 in the morning rather than evening may help. Typically mild and transient.

Fatigue or drowsiness
UncommonMild

Reported in some clinical trial participants. Usually mild and may improve with continued use.

Appetite changes
UncommonMild

Increased or decreased appetite reported. Monitor nutritional intake.

Anxiety or restlessness
RareMild

Rare in clinical trials. May indicate dose adjustment needed. Consult healthcare provider if persistent.

Elevated liver enzymes
RareModerate

Rare reports in clinical trials. Requires monitoring via liver function tests. Discontinue if significant elevation occurs and consult healthcare provider.

Dosing Reference

ParameterValue
Dose range20-40 mg
Frequency1x daily
TimingMorning with food
RouteOral

Oral administration only. NSI-189 is a small-molecule compound, not a peptide. Start at 20 mg and titrate based on tolerance. Take with food to improve absorption and reduce gastrointestinal upset. Research compound — not approved for human use outside clinical trials.

Research disclaimer

Figures drawn from published research literature and community logs. Not clinical recommendations. Consult a qualified professional. Research use only.

Reconstitution Guide

Do not use saline or bacteriostatic saline — use only bacteriostatic water for reconstitution

Do not shake the vial vigorously; gentle swirling prevents peptide degradation

Discard immediately if the solution appears cloudy, discolored, or contains visible particles

Use within 30 days of reconstitution when stored at 2–8°C

Do not freeze the reconstituted solution; freezing may denature the peptide

Use the PeptideVolt reconstitution calculator for your exact concentration

Use the PeptideVolt reconstitution calculator for your exact concentration

Molecular and Pharmacological Data

Molecular weight272.36 g/mol
Half-lifeApproximately 8-12 hours (oral bioavailability varies)

NSI-189 is a small-molecule benzylpiperazine-aminopyridine compound that promotes hippocampal neurogenesis by stimulating neural progenitor cell proliferation and differentiation. It increases hippocampal volume and enhances cognitive function through activation of neurotrophic signaling pathways, particularly BDNF-mediated mechanisms. These neuroprotective and neurogenic effects underlie its potential antidepressant and cognitive-enhancing properties.

Hippocampal Neurogenesis

NSI-189 promotes the proliferation and differentiation of neural progenitor cells in the dentate gyrus of the hippocampus, leading to increased production of new neurons. This neurogenic effect is associated with improved cognitive function and mood regulation.

BDNF Signaling

NSI-189 enhances brain-derived neurotrophic factor (BDNF) signaling, which supports neuronal survival, growth, and synaptic plasticity. BDNF activation is linked to antidepressant effects and cognitive enhancement.

Neuroprotection

NSI-189 provides neuroprotective effects through activation of survival signaling pathways, reducing neuroinflammation and oxidative stress in the brain.

  • NSI-189 is a small-molecule compound, not a peptide or protein
  • Increases hippocampal volume in clinical trials — measurable by MRI
  • Promotes adult neurogenesis in the dentate gyrus
  • Enhances BDNF signaling and neurotrophic support
  • Oral bioavailability improved when taken with food
  • Phase 2 clinical trials demonstrated safety and efficacy for depression and cognitive enhancement

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Research Use Only. All content on this page is provided for informational and educational purposes related to scientific research. NSI-189 is not approved for human use by the FDA or any equivalent regulatory body. This is not medical advice. Do not use any substance discussed here for therapeutic, diagnostic, or preventative purposes. Consult a qualified healthcare professional before making any health-related decisions. The Peptide Volt does not endorse the use of any research chemicals. 18+ only.